Understanding Impedance Spectra

The cell layer's resistance TER and capacitance Ccl contribute predominantly at mid-range frequencies to the total impedance, leading to the formation of a plateau. This characteristic permits to separate these two contributions of interest from the peripheral impedances dominating the low and high frequency end of the spectrum. It is instructive to consider how changes in resistance TER or capacitance Ccl affect the measured impedance spectrum. The following diagrams show that - as a rule of thumb - the resistance TER determines the height and the capacitance Ccl the width of the plateau.
 cellZscope An increase in the resistance TER shifts the plateau upwards.
 cellZscope
An increase in the capacitance Ccl narrows the plateau.