Hardware

The cellZscope measures the impedance of barrier-forming cell cultures grown on permeable membranes. It is fully compatible with a broad range of standard cell culture inserts from different manufacturers, e.g. BD Biosciences, Corning, Greiner Bio-One, Millipore, and Nunc. The cell module can be loaded with a maximum of 24 inserts simultaneously.

Three versions of the module are available which are optimized for use with small ("24-well" type), mid-sized ("12-well" type), or large ("6-well" type) inserts, respectively. During an experiment the cell module is typically placed inside a standard incubator while the external controller is connected to a computer running the cellZscope software.

cellZscope - Hardware

The design of the cell module warrants easy handling also under an airflow work bench. The two top covers can separately be lifted to gain free access to the wells for loading with standard cell culture inserts.

cellZscope - Hardware

The inner and outer compartments can be reached with a pipette for convenient media exchange, cell seeding and / or adding of substances. Both the apical and the basolateral side of the cell layers remain fully accessible throughout the experiments.

cellZscope - Hardware

The hinged lids can be opened without having to remove the electrodes from the wells while an experiment is running.

cellZscope - Hardware

Openings in the transparent cover give access to the inner and outer compartment of each well. Medium can easily be exchanged and substances added with a pipette.

Software

The cellZscope comes with customized software which provides full instrument control including user defined experiment setup and automated data acquisition. Furthermore, it features comprehensive tools for displaying and statistically analyzing data as well as printing and exporting results.

The user interface is geared to easy operation of the instrument. It allows to

  • schedule measurements by specifying time intervals for data acquisition according to experimental requirements,
  • monitor results in real time,
  • enter individual well descriptions and notes,
  • log and annotate all experimental events.
cellZscope - Software
Experiment setup and data acquisition

The cellZscope software allows to monitor and record all relevant parameters during the course of the experiment, in particular it provides the

  • transepithelial / -endothelial electric resistance TER and the
  • electric capacitance Ccl

of the cell layer as easy readout parameters.

cellZscope - Software
Data display and statistical analysis
cellZscope - Software
Export of data and graphics by simple "drag and drop" or "copy and paste"

Corning is a registered trademark of Corning Inc. Millipore is a registered trademark of Millipore Corp. Greiner is a registered trademark of Greiner Bio-One GmbH. BD is a trademark of Becton, Dickinson and Company. Nunc is a trademark of Thermo Fisher Scientific Inc. All other brand or product names are trademarks or registered trademarks of their respective holders.