cellZscopeE

cellZscope E

The cellZscopeE is our entry model for automated TER measurement under physiological conditions. Main features are:

  • Automatic determination of TER
  • Full compatibilty with a broad range of standard cell culture inserts
  • Cell Module can be loaded with 6 inserts simultaneously
  • Time resolution per well: 1 datapoint/hour
  • Upgrade to cellZscope+ possible

Details

The cellZscopeE is the ideal entry-level system for computer-controlled TER (transepithelial / -endothelial resistance) measurements. TER of the cell layers under investigation is determined in real-time. Reliable long term measurements over days and weeks can be carried out.

The convincing performance and instrument operations and easy maintenance make the cellZscopeE the ideal tool for controlling the confluence of cell layers as well as for studying the influence of substances on the barrier function. It sweeps over a wide frequency range instead of simply measuring at a very few frequency points. This ensures a reliable and unambiguous detection of cell layer properties.

cellZscope - Hardware

The innovative design of the cellZscopeE provides investigators full apical and basolateral access to all wells. Even without the user has to fully open the cell module. An aperture in the cover gives access to the inner and outer compartment of each well. Medium can easily be exchanged and substances added with a pipette.  

Single-piece stainless steel “pots” serve as bottom electrodes, as vessels for holding the cell culture medium, and as supports for cell culture inserts.

cellZscope - Hardware

The lid is made of highest quality stainless steel not only providing superior durability but also excellent compatibility with all standard cleaning and sterilization methods. The bottom electrodes can be detached easily without the use of tools. These features make the cellZscope systems a snap to clean and sterilize.

cellZscope - Hardware

Three well sizes are available for use with small (“24-well” type), mid-sized (“12-well” type), or large (“6-well” type) inserts, respectively.

Different sizes of cell culture inserts can be combined within a single Cell Module. Thus allowing the automated monitoring and direct comparison of the barrier properties of cell layers housed in up to three different sized cell culture inserts.

Applications

Software

The cellZscope comes with customized software which provides full instrument control including user defined experiment setup and automated data acquisition. Furthermore, it features comprehensive tools for displaying and statistically analyzing data as well as printing and exporting results.

cellZscope - Software

The user interface is geared to easy operation of the instrument. Main features are:

  • Monitor results in real time,
  • enter individual well descriptions and notes,
  • log and annotate all experimental events.
cellZscope - Software

The cellZscope software allows to monitor the

  • transepithelial / -endothelial electric resistance TER

of the cell layer as easy readout parameter during the course of the experiment.

cellZscope - Software

User-friendly functions for exporting cellZscope data into popular file formats are included, making seminar and manuscript preparations a breeze.

Upgrade Options

Owner of the cellZscopeE do have the opportunity to upgrade their system.

There are two ways to improve a cellZscopeE system (see diagram below). Upgrade A affects the measurement electronics and the software. The Controller will be equipped with an additional module. This results in an additional output parameter (cell layer capacitance Ccl), an enhanced measurement performance and extra software features. The Controller and software are then similar to those of the cellZscope+.

Upgrade B includes all improvements coming with Upgrade A and an additional Cell Module with 24 individual wells (cellZscope+ Cell Module). This results in a full cellZscope+ system. And of course the cellZscopeE Cell Module also remains at the customer site.

Upgrade Options cellZscopeE

Detailed Upgrade Options

  cellZscope E Upgrade A Upgrade B
Output parameters TER TER, Ccl TER, Ccl

Enhanced measurement performance, icluding:
- Increased time resolution
- Freely customizable measurement intervals
- Measurement frequency range adjustable

 
Additional software features, including:
- Raw data accessible
- Additional options for data fitting
 
cellZscopeE Cell Module with 6 wells
cellZscope+ Cell Module with 24 wells    

 

Compatible cell culture inserts

cellZscope - well sizes

The cellZscopeE is compatible with a variety of standard cell culture inserts from different manufacturers. Three versions of the electrodes are available. They are tailored for use with small (“24-well” type), mid-sized (“12-well” type), or large (“6-well” type) inserts, respectively. Different well sizes can be combined within one Cell Module.

 

cellZscope - compatible inserts
Some examples of compatible cell culture inserts (nanoAnalytics GmbH assumes no responsibility or liability for any errors or inaccuracies).


cellZscope is a registered trademark of nanoAnalytics GmbH. Corning, Transwell and Falcon are registered trademarks of Corning Inc. Greiner Bio-One is a registered trademark and ThinCert is a trademark of Greiner Bio-One GmbH. Millipore and Millicell are registered trademarks of Merck KGaA. Nunc is a trademark of Thermo Fisher Scientific Inc. All other brand or product names are trademarks or registered trademarks of their respective holders.

Specifications

Dimensions (L×W×H)

Controller 26 cm × 6 cm × 27 cm
(10.2 in × 2.4 in × 10.6 in)
Cell Module 18 cm × 15 cm × 7 cm
(7.1 in × 6.0 in × 2.8 in)
Space required in incubator 26 cm × 20 cm × 9 cm
(10.2 in × 7.9 in × 3.6 in)

Connector Cable

Length 150 cm (59 in)
Cross section (W×T) 15 mm × 4 mm
(0.6 in × 0.16 in)
Usually the ribbon cable can be simply fed through the front door of the incubator.

Measurement Parameters

Frequency range 1 Hz to 100 kHz
Measurement time for 6 wells ~ 5 min
Time resolution per well 1 datapoint/hour

Cell Module

Number of wells 6
6-well size
12-well size
24-well size

Miscellaneous

Power Supply 100 – 240 Vac; 0.8 A; 47 – 63 Hz
Minimum requirements for the control computer - 1.0 GHz processor speed
- 1024 MB RAM
- USB 2.0 port
- 1024×768 display
Compatible OS Microsoft Windows® 7/8/10