High-speed TEER monitoring of MDCK I cells
during lysis due to saponin treatment
Inadequate temporal resolution can mean that effects or temporary changes cannot be measured and observed. The cellZscope3 can record these fast processes with a temporal resolution of one data point per 30 s in up to 96 parallel wells.
In the experiment shown here, MDCK-I cells were cultivated on inserts in the cellZscope. The experiment. It was waited until a confluent monolayer was formed and the TEER reached a constant value. The cells were then exposed to different concentrations of Saponin.
Saponin is often used as a model substance / positive control, e.g. when examining substances that can lead to cell membrane lysis. If a certain substance causes a lysis of the cell-membrane, a sufficiently good time resolution is sometimes necessary in order to be able to monitor dose-dependent differences in the barrier dynamics.
TEER/TEER0h vs. time of MDCK-I cells seeded with a cell density of 495000 cells/cm2. At time t = 0 min, Saponin was added at different concentrations; Orange: control (L15 medium), light blue: 20 µg/ml Saponin, blue: 50 µg/ml Saponin, dark blue: 100 µg/ml Saponin.
With the cellZscope3, these fast processes can be observed without having to forego comprehensive impedance analysis. Measuring a broad frequency spectrum ensures that cell layer properties (TEER and capacitance) are unambiguously and accurately determined.
nanoAnalytics thanks Tobias Naber and J. Wegener (Universität Regensburg) for their comprehensive support of this study.